C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12Q 1/18 (2006.01) G01N 21/64 (2006.01)
Patent
CA 2066329
This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's fluorescent emission in a concentration dependent manner. Respiring microorganisms will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification and metabolic activity of microorganisms by determining their effect on the oxygen concentration of the media in which they are present.
Burrell Gregory J.
Hu Kwok-Yu
Monthony James F.
Sapitowicz Robert
Stitt David T.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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