Microbial process for production of enzymes

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01) C07K 14/37 (2006.01) C12N 9/20 (2006.01)

Patent

CA 2671797

The present invention relates to a suspension culture process for the production of an enzyme, comprising the steps of providing a minimal medium which contains deionized water, mineral salts and an organic carbon source in a vessel; inoculation of the minimal medium with a fungus; incubation of the minimal medium at a pH of 1-4 for a period which is sufficient for the fungus to be optically visible in the minimal medium, and also obtaining the enzyme, wherein the minimal medium and the vessel are not sterilized. In addition, the invention relates to an enzyme which can be produced by the process according to the invention.

La présente invention concerne un procédé immergé pour produire une enzyme, comprenant les étapes consistant à introduire dans un récipient un milieu minimum contenant de l'eau déminéralisée, des sels minéraux et une source de carbone organique; ensemencer le milieu minimum avec un champignon; incuber le milieu minimum à un pH de 1 à 4 pendant une durée suffisante pour que le champignon soit optiquement visible dans le milieu minimum; et récupérer l'enzyme. Dans ce procédé, le milieu minimum et le récipient ne sont pas stérilisés. L'invention concerne en outre une enzyme qui peut être produite par le procédé de l'invention.

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