C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/86, 195/1.2,
C12N 15/00 (2006.01) C09B 7/02 (2006.01) C12N 15/52 (2006.01) C12P 17/16 (2006.01)
Patent
CA 1217155
- 20 - ABSTRACT "MICROBIAL PRODUCTION OF INDIGO" Microbial synthesis of indigo dyestuff in indole-free media is disclosed. Indigo production is preferably accomplished by genetic transformation of selected host cells having the capacity to produce and accumulate indole (either as a result of endogenous genomic capacity or genetic transformation) to incor- porate the capacity for synthesis of an aromatic dioxy- genase enzyme. Growth of transformed cells under suitable conditions facilitates aromatic dioxygenase enzyme catalyzed oxidative transformation of cellular indole, with consequent formation of indigo from the oxidized reaction products. In a highly preferred embodiment, E.coli cells having endogenous indole production capacity are transformed with a DNA expres- sion vector comprising the structural gene for naph- thalene dioxygenase, resulting in the microbial synthesis of isolatable quantities of indigo.
439717
Amgen
Ridout & Maybee Llp
LandOfFree
Microbial production of indigo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Microbial production of indigo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microbial production of indigo will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1317554