C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/131
C12P 7/62 (2006.01) C07C 59/01 (2006.01) C12P 7/40 (2006.01) C12P 7/42 (2006.01)
Patent
CA 1100896
ABSTRACT OF THE DISCLOSURE D(-)-3-Hydroxybutyric acid occurs in humans, animals plants and microorganisms and thus could be useful in parenteral nurtition and as a starting material in the manufacture of chemical compounds which are com- patible with the human organism. The invention relates to a process of manu- facturing D(-)-3-hydroxybutyric acid, which comprises subjecting a micro- organism capable of separating out butyric acid or of accumulating poly-(D-3- hydroxybutyric acid) to the action of mutagenic agents; selecting from the mutants thus obtained those which cannot metabolize D(-)-3-hydroxybutyric acid; further selecting from the thus selected mutants those which, option- ally after further treatment with muuagenic agents, are capable of producing at least 100 mg of D(-)-3-hydroxybutyric acid per litre of the nutrient medium within 30 hours when grown at a temperature of approximately 24-40°C and a pH value of approximately 4-8 in an aqueous nutrient medium which con- tains a carbon source selected from carbon dioxide, an assimilable carbo- hydrate from the group glucose, fructose, saccharose, lactose, molasses and whey, an assimilable acohol from the group methanol, ethanol and glycerin and a spent lye from caprolactam synthesis, together with an assimilable nitrogen source and trace elements, breeding a thus obtained microorganism in the above said nutrient medium and under the above said culture conditions, and isolating the D-(-)-3-hydroxybutic acid thereby produced from the culture medium.
283963
Agroferm Ag
Fetherstonhaugh & Co.
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