Microbiological process for the preparation of a protein by...

C - Chemistry – Metallurgy – 12 – N

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195/1.2, 195/1.2

C12N 15/70 (2006.01) C07K 14/61 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1304021

IN THE CANADIAN PATENT & TRADEMARK OFFICE PATENT APPLICATION entitled: Microbiological process for the preparation of a protein by culturing a mutant bacterial strain in the name of: LEGOUX Richard LEPLATOIS Pascal LIAUZUN JOSEPH Evelyne NIAUDET Brigitte ROSKAM Willem Assignee: Société Anonyme styled- SANOFI ABSTRACT OF THE DISCLOSURE The invention relates to a microbiological process for the preparation of a protein by culturing a mutant bacterial strain. This process consists in using a bacterial strain carrying a mutation which limits, or even suppresses, the expression of the operons whose transcription depends on the fixation of the cAMP-CRP complex, a DNA sequence coding for a protein precursor having been introduced into the said bacterial strain. Application: production of hGH.

534359

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