Microbiological process for the production of aromatic...

C - Chemistry – Metallurgy – 12 – P

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C12P 17/12 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2084456

ABSTRACT OF THE DISCLOSURE A microbiological process is disclosed for the production of hydroxy-heterocyclic carboxylic acid of the formula: Image (I) wherein R1 represents a hydrogen or halogen atom and X represents a nitrogen atom or a CR2 function in which R2 is a hydrogen or halogen atom, starting from the corresponding heterocyclic carboxylic acid. The process is performed by first cultivating an aerobic biomass which utilizes nicotinic acid, with nicotinic acid and a mineral acid in a molar ratio of nicotinic acid to mineral acid of from 1 to 8, this ratio being maintained throughout the entire cultivation phase. Then, the hydroxylation of the corresponding heterocyclic carboxylic acid of the general formula: Image (II) wherein R1 and X have the above-mentioned meanings, is performed with the biomass. Under these conditions, microorganisms of the species Pseudomonas acidovorans DSM 7205, Pseudomonas acidovorans DSM 7203, Alcaligenes faecalis DSM 7204 and microorganisms with the designation DSM 7202 are preferably concentrated in the cultivation phase.

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