Microbiological scaled sterility test strips

Details Microbiological scaled sterility test strips Microbiological scaled sterility test strips

C - Chemistry, Metallurgy

12

M

150/16.2

C12M 1/00 (2006.01) C12M 1/20 (2006.01) C12N 5/00 (2006.01) C12Q 1/22 (2006.01)

Patent

CA 1077815

ABSTRACT OF THE DISCLOSURE A method and apparatus for obtaining a permanent visual record of the microbiological effectiveness of a sterilizing cycle is provided by a spore carrier strip having a plurality of indivi- dual spore colony sites defined on the surface thereof each of said sites containing a different population of spores, as for example, 102, 103, 104, 105, and 106. The spore carrier is ex- posed to the sterilizing cycle, then incubated in a sterile nutrient culture medium containing a color indicator for viable microorgan- isms such as 2,3,5-triphenyl tetrazolium chloride. The develop- ment or lack of development of color on each spore colony site of successively increasing spore populations indicates the degree of effectiveness of the sterilizing cycle. The developed color is permanent and the carrier strip may be retained to provide a per- manent visual record of the effectiveness of the sterilizing cycle.

277424

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