Microbiologically produced n-acyl-l-proline acylase, process...

Details Microbiologically produced n-acyl-l-proline acylase, process... Microbiologically produced n-acyl-l-proline acylase, process...

C - Chemistry, Metallurgy

12

N

195/123, 195/134

C12N 9/80 (2006.01) C12P 13/24 (2006.01)

Patent

CA 2024622

ABSTRACT OF THE DISCLOSURE The present invention relates to a microbiologically produced thermostable N-acyl-L-proline aylase and to its production from Cornanonas tectosteroni DSM 5416 or Alcaligenes denitrificans DSM 5417. The enzyme can be used for the production of L-proline from N-chlore-acetyl, D,L-proline, N-formyl-D,L-prollne, N- proplonyl-D,L-proline, N-butyryl-D,L-proline, N-valeryl-D,L- proline, N-caproyl-D,L-proline, of L-thiazolidine-4-carboxylic acid from N-acetyl-D,L-thiazolidine-2-carboxylic acid or N- chloro-acetyl-D,L-thiazolidine 2-carboxylic acid and L- pipecolinic acid from N-acetyl-D,L-pipecolinic acid or N-chloro- acetyl-D,L-picolinic acid. Furthermore, the enzyme can be used for the productlon of N-acetyl-L-proline, N-propionyl-proline and N-brityrl-L-proline from L-prolin and the corresponding carboxylic acid.

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