Microfabricated detection structures

Details Microfabricated detection structures Microfabricated detection structures

G - Physics

01

N

G01N 33/543 (2006.01) B01D 61/18 (2006.01) B01D 67/00 (2006.01) B01D 71/02 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) B01L 7/00 (2006.01) C12M 1/26 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12M 3/00 (2006.01) C12M 3/08 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 1/28 (2006.01) G01N 35/00 (2006.01) A61B 17/04 (2006.01) B01F 5/06 (2006.01)

Patent

CA 2134478

Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port (16), and a mesoscale flow system that includes a sample flow channel (20) extend- ing from the inlet port. The mesoscale flow system further includes an analyte detection region (22) in fluid communication with the flow channel (20) comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyle. The binding moiety may be immobilized in the detection region. The mecoscale detection systems of the invention may be used in a wide range of applica- tions, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.

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