C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) C12M 1/00 (2006.01)
Patent
CA 2174140
An improved microfabricated apparatus for conducting a multiplicity of individual and simul- taneous binding reactions is described. The appara- tus comprises a substrate on which are located dis- crete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and ter- minate on a second surface thereof such that when a test sample is allowed to the substrate, it is capa- ble of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.
La présente invention concerne l'amélioration d'un dispositif à microstructure destiné la réalisation de multiples réactions individuelles et simultanées de liaison. Ce dispositif se compose d'un substrat pourvu d'emplacements discrets et isolés propices aux réactions de liaison. Le dispositif se caractérise par des zones discrètes et isolées qui traversent ledit substrat et débouchent au niveau de sa seconde surface pour que, lors de l'exécution d'un test sur échantillon au niveau du substrat, l'échantillon puisse pénétrer au travers des différentes zones au cours du déroulement de ladite réaction de liaison. Ce dispositif convient particulièrement aux opérations de séquencement des molécules d'ADN par hybridation.
Houston Advanced Research Center
Lockheed Martin Energy Research Corporation
Smart & Biggar
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