C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) B01L 7/00 (2006.01) B81B 1/00 (2006.01)
Patent
CA 2276251
Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated substrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.
Procédés et compositions permettant l'amplification isotherme d'acides nucléiques dans un substrat microfabriqué. Des procédés et compositions destinés à l'analyse d'acides nucléiques amplifiés de manière isotherme sont également décrits. Les substrats microfabriqués et les procédés de détection et d'amplification isotherme décrits sont destinés à être utilisés dans différentes méthodes diagnostiques, en particulier celles associées aux maladies se caractérisant par une modification des séquences géniques ou de l'expression génique.
Beyer Wayne F. Jr.
Burke David T.
Burns Mark
Denuzzio John D.
Johnson Brian N.
Becton Dickinson And Company
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
The Regents Of The University Of Michigan
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