Microfluidic cell culture device

Details Microfluidic cell culture device Microfluidic cell culture device

C - Chemistry, Metallurgy

12

M

C12M 1/00 (2006.01) C12N 5/071 (2010.01) C12M 3/00 (2006.01) C12N 1/00 (2006.01)

Patent

CA 2632422

Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.

L'invention concerne des dispositifs microfluidiques destinés à une culture cellulaire, et des méthodes pour utiliser ces dispositifs. Un dispositif de l'invention comprend un substrat et une membrane. Le substrat comprend un réservoir en communication fluidique avec un passage. Un fluide biocompatible peut être ajouté dans le réservoir et dans le passage. Le réservoir est conçu pour recevoir et pour retenir au moins une partie d'une masse cellulaire. La membrane sert de barrière contre l'évaporation du fluide biocompatible situé dans le passage. Un fluide couvrant peut être ajouté pour couvrir le fluide biocompatible afin d'éviter l'évaporation du fluide biocompatible.

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