Microfluidic device and method of using same

G - Physics – 01 – N

Patent

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Details

G01N 1/28 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2600899

The invention relates to a microfluidic device comprising one or more fluid channels, one or more fluid ports, and a V-shaped particle retention structure. The fluid channel is generally opposite the particle retention structure, fluid ports are located between the fluid channel and the particle retention structure, and the particle retention structure has sloped side walls. Fluid, including reagents, can be delivered to the microfluidic device through the one or more fluid channels or the fluid ports. The invention also relates to methods of using the microfluidic device to monitor, observe, measure, or record a biological parameter of a particle, to separate a single particle from a group of particles, to culture a cell, to treat a particle, and to move a particle back and forth in the device.

La présente invention a trait à un dispositif microfluidique comportant un ou des canaux de fluide, un ou des orifices de fluide, et une structure en V de retenue de particules. Le canal de fluide est globalement en face de la structure de retenue de particules, des orifices de fluide sont situés entre le canal de fluide et la structure de retenue de particules, et la structure de retenue de particules comporte des parois latérales inclinées. Un fluide, comprenant des réactifs, peut être alimenté au dispositif microfluidique à travers ledit un ou lesdits plusieurs canaux de fluide ou les orifices de fluide. L'invention a également trait à des procédés d'utilisation du dispositif microfluidique pour le contrôle, l'observation, la mesure, ou le relevé d'un paramètre biologique d'une particule, pour l'isolement d'une particule à partir d'un groupe de particules, pour la culture d'une cellule, pour le traitement d'une particule, et pour le déplacement d'une particule en va-et-vient dans le dispositif.

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