Microfluidic device and methods of using same

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/36 (2006.01) B01L 3/00 (2006.01) C12N 11/16 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) F04B 43/02 (2006.01) G01N 15/06 (2006.01) G01N 35/00 (2006.01) B01F 13/00 (2006.01)

Patent

CA 2467587

A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

La présente invention concerne divers dispositifs microfluidiques à base d'élastomère ainsi que des procédés d'utilisation et de fabrication de ces dispositifs. Certains dispositifs comportent des réseaux de sites de réaction qui facilitent les analyses à rendement élevé. Certains dispositifs comprennent également des sites de réaction situés à l'extrémité des canaux fermés au niveau desquels les réactifs on été préalablement déposés lors de la fabrication. Les réactifs se mettent en suspension dès que l'échantillon est introduit dans le site de réaction. Ces dispositifs peuvent être utilisés avec divers dispositifs de chauffage et peuvent par conséquent être utilisés dans diverses analyses nécessitant une régulation de la température, y compris des applications de thermocyclage telles que des réactions d'amplification d'acide nucléique, le génotypage et les analyses d'expression génique.

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