Microfluidic device for purifying a biological component...

G - Physics – 01 – N

Patent

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G01N 1/34 (2006.01) B01L 3/00 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2621632

A method of purifying a biological component found in a biological sample by extracting the biological component from the biological sample. The method is performed using a microfluidic device having at least one well for receiving the biological sample and at least one channel for introducing and removing fluids. A plurality of magnetic beads having a factor with an affinity for the biological component is introduced to the well together with a suitable biological sample. The biological sample is manipulated to release the biological component in proximity to the magnetic beads which are then segregated within the well while removing the biological sample. An elution solution for the biological component is introduced to the well and the elution solution together with the biological component are withdrawn therefrom.

La présente invention concerne un procédé de purification d'un constituant biologique trouvé dans un échantillon biologique et extrait de ce dernier. Le procédé est effectué au moyen d'un dispositif microfluidique comportant au moins un puits destiné à recevoir l'échantillon biologique et au moins un passage permettant d'introduire et de faire sortir les fluides. Une pluralité de billes magnétiques présentant un facteur d'affinité envers le constituant biologique est introduite dans le puits en même temps qu'un échantillon biologique approprié. L'échantillon biologique est manipulé pour libérer le constituant biologique à proximité des billes magnétiques qui sont ensuite séparées dans le puits alors qu'on retire l'échantillon biologique. Une solution d'élution pour le constituant biologique est introduite dans le puits, la solution d'élution et le constituant biologique étant ensuite enlevés dudit puits.

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