Microfluidic devices and methods for chemical assays

B - Operations – Transporting – 01 – L

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Details

B01L 3/00 (2006.01) B01F 11/00 (2006.01) B01F 13/00 (2006.01) B01J 19/00 (2006.01) F16K 1/00 (2006.01) F16K 7/12 (2006.01)

Patent

CA 2433145

An assay device in which to carry out a fluid-phase chemical assay, comprising means for supporting a test substrate, a sample chamber for receiving a fluid sample and at least one fluid control device for controlling the movement of fluid into and/or out of the sample chamber, wherein the fluid control device comprises a fluid outlet chamber in fluid communication with the sample chamber, and a displaceable flexible diaphragm the displacement of which alters the volume of the outlet chamber so as to allow and/or restrict fluid flow between the outlet and sample chambers. The invention also provides assay apparatus incorporating such a device, an assay station for use as part of such apparatus, a fluid control unit for use as part of the assay device and a method of conducting an assay which may involve the use of such apparatus and devices.

L'invention concerne un dispositif d'analyse permettant d'effectuer une analyse chimique en phase fluidique, qui comprend des moyens de support de substrat d'essai, une chambre d'échantillonnage destinée à recevoir un échantillon fluidique, et au moins un dispositif de régulation fluidique permettant de réguler le déplacement d'un fluide dans la chambre d'échantillonnage et/ou à l'extérieur de celle-ci. Le dispositif de régulation fluidique comprend une chambre de sortie fluidique en communication fluidique avec la chambre d'échantillonnage, et une membrane souple mobile dont le déplacement modifie le volume de la chambre de sortie de façon à autoriser et/ou restreindre l'écoulement fluidique entre les chambres de sortie et d'échantillonnage. L'invention concerne également un appareil d'analyse incorporant ledit dispositif, une station d'analyse utilisée comme partie de ce dispositif, une unité de régulation fluidique utilisée comme partie du dispositif d'analyse, et un procédé permettant d'effectuer une analyse qui implique l'utilisation de l'appareil et des dispositifs.

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