Microfluidic separation column devices and fabrication methods

B - Operations – Transporting – 01 – L

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B01L 3/00 (2006.01) B01F 13/00 (2006.01) B01J 19/00 (2006.01) B29C 65/00 (2006.01) G01N 30/60 (2006.01) G01N 30/56 (2006.01)

Patent

CA 2472945

Pressure-driven microfluidic separation devices, such as may be used for performing high performance liquid chromatography, are provided. Multiple separation columns may be defined in a single device and packed with stationary phase material retained by porous frits. One or more splitters may be provided to distribute slurry and/or mobile phase among multiple separation columns. In one embodiment, separation devices are substantially planar and fabricated with multiple device layers. Systems and methods employing slurry for packing separation devices are also provided.

L'invention concerne des dispositifs de s~paration microfluidique ~ pression, par exemple pour la chromatographie en phase liquide haute performance. On peut d~finir plusieurs colonnes de s~paration dans un dispositif unique, ces colonnes ~tant garnies de mat~riau en phase stationnaire retenu par de la fritte poreuse. Il est possible de pr~voir un ou plusieurs diviseurs pour r~partir la suspension ~paisse et/ou les ~l~ments en phase mobile entre les diff~rentes colonnes de s~paration. Selon une variante, les dispositifs de s~paration consid~r~s sont sensiblement plats, et ils comportent plusieurs couches constitutives. L'invention concerne enfin des syst­mes et des proc~d~s relatifs ~ l'utilisation de suspension ~paisse pour des dispositifs de s~paration ~ garnissage.

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