Microfluidic system for analysis of nucleic acids

Details Microfluidic system for analysis of nucleic acids Microfluidic system for analysis of nucleic acids

B - Operations, Transporting

01

L

B01L 3/00 (2006.01) G01N 1/28 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2504516

A system (10) is provided, including apparatus and methods, for microfluidic processing and/or analysis of a nucleic acid(s) (127) in a sample having the nucleic acid(s) and waste material. The system (10) includes a microfluidic device (14) having a fluid-handling portion (42) and an assay portion (44). The fluid-handling portion (42) may be configured to move fluid mechanically and defines at least one fluid compartment (54). The fluid-handling portion (42) is configured to receive the sample and to pre-process the sample in the fluid compartment (54) to at least partially separate the nucleic acid (127) from the waste material. The assay portion (44) interfaces with the fluid- handling portion (42) and defines at least one fluid chamber. The fluid chamber is connected fluidically to the fluid compartment (54). The assay portion (44) includes electronics (58) configured to process the nucleic acid (127) in the fluid chamber.

L'invention concerne un système (10), comprenant appareil et procédés, de traitement microfluidique t/ou d'analyse d'acide(s) nucléique(s) (127) dans un échantillon comprenant des acides nucléiques et un matériau déchet. Le système (10) comprend un dispositif microfluidique (14) comportant une partie de manipulation de fluide (42) et une partie test (44). La partie de manipulation de fluide (42) peut être configurée afin de déplacer mécaniquement le fluide et définit au moins un compartiment fluide (54). Elle (42) est configurée afin de recevoir l'échantillon et de prétraiter cet échantillon dans le compartiment de fluide (54) afin de séparer au moins partiellement l'acide nucléique (127) du matériau déchet. La partie test (44) est interfacée avec la partie de manipulation de fluide (42) et définit au moins une chambre de fluide. La chambre de fluide est connectée, par voie fluidique, au compartiment de fluide (54). La partie test (44) contient l'électronique (58) conçue afin de traiter l'acide nucléique (127) dans la chambre de fluide.

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