Microgels for use in medical diagnosis and methods of making...

G - Physics – 01 – N

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G01N 1/28 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2202914

An electrophoresis microgel is formed in a gel holder. The gel holder comprises a top substrate, a bottom substrate and a spacer disposed between the top substrate and the bottom substrate. The spacer establishes a separation of from 25 to 250 microns between the top substrate and the bottom substrate. A gel compartment is formed by partially sealing the top substrate to the bottom substrate, while leaving an opening for the introduction of unpolymerized gel. The gel compartment is then filled with an unpolymerized gel, which is polymerized in the gel compartment. Electrodes may be printed on the substrates, may be contacted to an exposed edge of gel, or may be applied through windows cut into one of the substrates. One type of gel holder makes use of graded beads having a diameter of 25 to 250 microns slurried in an adhesive such as an acrylate adhesive as the spacer. The slurry is printed onto the surface of one or both substrates to form a spacer of the desired shape, and then hardened using heat or light. If desired, the spacer can establish lanes within the gel.

Un microgel d'électrophorèse est formé dans un support. Le support du gel comporte un substrat supérieur, un substrat inférieur et un élément d'espacement placé entre le substrat supérieur et le substrat inférieur. Cet élément d'espacement crée une séparation de 25 à 250 microns entre le substrat supérieur et le substrat inférieur. On forme un compartiment pour gel en scellant partiellement le substrat supérieur sur le substrat inférieur. Le compartimement est ensuite rempli d'un gel non polymérisé, sa polymérisation étant effectuée dans le compartiment. Des électrodes peuvent être imprimées sur les substrats, être mises en contact avec un bord exposé du gel ou être appliquées dans des fenêtres découpées dans un des substrats. Dans un type de support de gel, on trouve des billes calibrées d'un diamètre compris entre 25 et 250 microns qui sont en suspension dans une colle telle qu'une colle acrylate formant l'élément d'espacement. La suspension est imprimée sur la surface d'un des deux substrats afin de former un élément d'espacement de la forme désirée, puis est durcie à la chaleur ou à la lumière. Si on le souhaite, l'élément d'espacement peut former des voies dans le gel.

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