Microinjection assembly and methods for producing transgenic...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/00 (2006.01) A01K 45/00 (2006.01) A01K 67/027 (2006.01) C12M 3/10 (2006.01) G01N 1/28 (2006.01)

Patent

CA 2437238

The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.

L'invention concerne un assemblage de micro-injection comprenant un microscope, un système de micro-injection comportant un micromanipulateur, une micropipette, un oscillateur piézo-électrique, et une unité de suivi macrographique à angle oblique. Elle concerne aussi des procédés de micro-injection du disque embryonnaire d'un oeuf d'oiseau, permettant de fournir à l'embryon un noyau transgénique, un spermatozoïde ou un acide nucléique isolé. L'ovule peut être rendu à une femelle oiseau aux fins de durcissement de coquille et de ponte d'un oeuf avec éclosion d'un oiseau transfecté.

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