Microinjection procedure for gene transfer in fish

Details Microinjection procedure for gene transfer in fish Microinjection procedure for gene transfer in fish

C - Chemistry, Metallurgy

12

N

195/1.36

C12N 5/10 (2006.01) C12N 15/89 (2006.01) C12N 15/90 (2006.01) C12N 5/06 (2006.01)

Patent

CA 1341553

A method is provided for transferring into a fish egg DNA of at least one gene foreign to the fish egg so as to integrate the foreign DNA stably into the fish egg genome. The method comprises: inserting a hollow needle into the fish egg, through the micropyle thereof, and injecting therethrough and into the fish egg a selected volume of a solution containing a plurality of copies of the foreign DNA. The fish egg may be fertilized and in the inactive state during the injection procedure and activated after injection is completed. The method is particularly suited to transferring freeze-resistant characteristics of icy water fish to salmonids.

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