Microorganism for the production of succinic acid

Details Microorganism for the production of succinic acid Microorganism for the production of succinic acid

C - Chemistry, Metallurgy

12

P

C12P 7/46 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2721392

The invention relates to an isolated genetically modified microorganism in which the gene IDH1 and at least one of the genes SDH2 and DIC1 are under the control of a first promoter that is repressed to a growth culture medium by means of a cultivation additive and is active in the absence of the cultivation additive. The genes that are part of the group comprising "PYC1, ACS1, CIT1, ACO1, ICL1, MSL1, and CIT2, optionally also MDH3" are constitutively active. The invention further relates to uses of such a microorganism, especially for producing succinic acid.

L'invention concerne un microorganisme isolé, génétiquement modifié, dans lequel le gène IDH1 et au moins un des gènes SDH2 et DIC1 se trouvent sous le contrôle d'un premier promoteur qui est réprimé dans un milieu de culture de croissance par un adjuvant de culture et qui est actif en l'absence de l'adjuvant de culture, les gènes du groupe "PYC1, ACS1, CIT1, ACO1, ICL1, MSL1 et CIT2, éventuellement aussi MDH3" ayant une activité constitutive. L'invention porte également sur des utilisations d'un tel microorganisme, notamment pour produire de l'acide succinique.

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