Microorganism quantitation and detection method and kit

Details Microorganism quantitation and detection method and kit Microorganism quantitation and detection method and kit

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2236842

A method wherein predetermined amounts of a microorganism of interest or the DNA or RNA thereof are used as the external standard; a fraction of the genome of said microorganism is extracted, revealed using a specific method or subjected to reverse transcription and/or amplification; and the DNA or RNA concentrations of the standard and target microorganisms or the target microorganism amplification products are compared with those of the external standard to determine values for the DNA or RNA concentration or the total microorganism concentration in each target microorganism sample. The method is useful for the quantitation and detection of all microorganisms.

Dans le procédé selon l'invention, on utilise en tant qu'étalon externe des quantités données du micro-organisme concerné ou de l'ADN ou ARN de ce micro- organisme; et après extraction, révélation au moyen d'une méthode spécifique ou rétrotranscription et/ou amplification d'une fraction du génome dudit micro- organisme, on effectue une comparaison des concentrations d'ADN ou d'ARN des micro-organismes étalon et cible ou des produits d'amplification des micro- organismes cible avec ceux de l'étalon externe, pour en déduire les valeurs de la concentration d'ADN ou d'ARN ou de la concentration en micro-organisme totale dans chaque échantillon de micro-organisme cible. Application à la quantification et la détection de tous les micro-organismes.

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