C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/21 (2006.01) C12N 1/00 (2006.01) C12N 15/00 (2006.01) C12P 7/02 (2006.01) C12P 7/18 (2006.01) C12N 9/00 (2006.01) C12N 15/52 (2006.01)
Patent
CA 2746952
An exemplary non-naturally occurring microbial organism having an isopropanol pathway includes at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol and includes a succinyl-CoA:3-ketoacid-CoA transferase. Another organism having a 4- hydroxybutryate pathway includes at least one exogenous nucleic acid including an acetoacetyl-CoA thiolase, a 3- hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA transferase, a phosphotrans-4- hydroxybutyrylase, and a 4-hydroxybutyrate kinase. An exemplary organism having a 1,4-butanediol pathway includes an acetoacetyl-CoA thiolase, a 3-Hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA reductase (aldehyde forming), and a 1,4-butanediol dehydrogenase. Such an organism further comprising an acetyl-CoA pathway. Any of the aforementioned organisms are cultured to produce isopropanol, 4- hydroxybutryate, or 1,4- butanediol.
La présente invention concerne un organisme microbien d'origine non naturelle exemplaire ayant une voie isopropanol qui comprend au moins un acide nucléique exogène codant pour une enzyme de la voie isopropanol exprimée en une quantité suffisante pour produire de l'isopropanol et comprend une succinyl-CoA:3-cétoacide-CoA transférase. La présente invention concerne en outre un autre organisme ayant une voie 4-hydroxybutryate comprenant au moins un acide nucléique exogène comprenant une acétoacétyl-CoA thiolase, une 3-hydroxybutyryl-CoA déshydrogénase, une crotonase, une crotonyl-CoA hydratase, une 4-hydroxybutyryl-CoA transférase, une phosphotrans-4-hydroxybutyrylase, et une 4-hydroxybutyrate kinase. Un organisme exemplaire ayant une voie 1,4-butanediol qui comprend une acétoacétyl-CoA thiolase, une 3-hydroxybutyryl-CoA déshydrogénase, une crotonase, une crotonyl-CoA hydratase, une 4-hydroxybutyryl-CoA réductase (formant de l'alcool), une 4-hydroxybutyryl-CoA réductase (formant un aldéhyde), et une 1,4-butanediol déshydrogénase. Un tel organisme comprend en outre une voie acétyl-CoA. L'un quelconque des organismes mentionnés ci-dessus est cultivé pour produire de l'isopropanol, du 4-hydroxybutyrate, ou du 1,4-butanediol.
Burgard Anthony P.
Burk Mark J.
Trawick John D.
Genomatica Inc.
Smart & Biggar
LandOfFree
Microorganisms and methods for conversion of syngas and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Microorganisms and methods for conversion of syngas and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microorganisms and methods for conversion of syngas and... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1986526