C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/19 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12P 7/40 (2006.01) C12P 7/46 (2006.01)
Patent
CA 2725549
A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.
L'invention concerne un organisme eucaryote ou procaryote apparaissant de manière non naturelle et comprenant une ou plusieurs interruptions génétiques apparaissant dans des gènes codant des enzymes qui entraînent une augmentation de la production en fumarate, malate ou acrylate dans l'organisme, lorsque l'interruption génétique réduit l'activité de l'enzyme. La ou les interruptions génétiques entraînent une production accrue d'acrylate dans l'organisme. Les organismes qui produisent l'acrylate présentent un passage d'acrylate qu'au moins un acide nucléique exogène codant une enzyme de passage d'acrylate exprime dans une quantité suffisante pour produire de l'acrylate, le passage d'acrylate comprenant une décarboxylase. L'invention concerne des procédés de production de fumarate, de malate et d'acrylate comprenant la mise en culture desdits organismes.
Burgard Anthony P.
Burk Mark J.
Pharkya Priti
Genomatica Inc.
Smart & Biggar
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