C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/20 (2006.01) C12P 33/00 (2006.01)
Patent
CA 2070720
Abstract Provision of microorganisms having an ability to convert, in high yields. precursors in high concentrations to 3.alpha.- hydroxy-7-keto-5.beta.-cholanic acid, 3.alpha.-hydroxy-7,12-diketo-5.beta.- cholanic acid or 3.alpha., 12.alpha.-dihydroxy-7-keto- 5.beta.-cholanic acid, each being useful as an intermediate for preparation of ursodeoxycholic acid, and provision of methods to produce these compounds in high yields using such microorganisms. Bacillus sp. TTUR 2-2, TTUR 4-1, TTUR 4-2, TTUR 2-M4, TTUR 2-M5, TTUR 2-M4-250 and TTUR 2- M4-294 having an ability to convert precursors to 3.alpha.-hydroxy-7-keto-5.beta.- cholanic acid, 3.alpha.-hydroxy-7,12-diketo-5.beta.-cholanic acid or 3.alpha., 12.alpha.- dihydroxy-7-keto-5.beta.-cholanic acid, and processes for producing these compounds by culturing these microorganisms in precursor-containing media.
Kawaide Hiroshi
Kimura Hiromi
Okamura Akio
Yamaura Takurou
Gowling Lafleur Henderson Llp
Mitsubishi-Tokyo Pharmaceuticals Inc.
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