C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0784 (2010.01) A61K 39/00 (2006.01) A61K 39/12 (2006.01) C12N 5/10 (2006.01) C12N 15/87 (2006.01) C12N 15/88 (2006.01) A61K 48/00 (2006.01)
Patent
CA 2392071
The present invention provides an effective method for the transfection of dendritic cells by non-viral methods. The present invention provides this benefit by incubating dendritic cells and a specified transfection agent. The transfection agent comprises a polynucleotide and microparticles, with the microparticles being comprised of biodegradable polymer and cationic detergent. The dendritic cells and transfection agent are incubated for a time sufficient to transfect the dendritic cells with the polynucleotide.
L'invention concerne un procédé efficace de transfection de cellules dendritiques par des méthodes non-virales. On atteint cet objectif en incubant des cellules dendritiques avec un agent de transfection particulier. Cet agent de transfection comprend un polynucléotide et des microparticules, ces dernières étant composées d'un polymère biodégradable et d'un détergent cationique. On incube les cellules dendritiques et l'agent de transfection pendant une durée suffisante pour transfecter les cellules dendritiques avec le polynucléotide.
Denis-Mize Kimberly Sue
Donnelly John James
Ott Gary Steven
Borden Ladner Gervais Llp
Chiron Corporation
Novartis Vaccines And Diagnostics Inc.
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