Microprojectile bombardment transformation of brassica

Details Microprojectile bombardment transformation of brassica Microprojectile bombardment transformation of brassica

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2629284

The invention relates to the genetic manipulation of plants, particularly plants of the genus Brassica. Methods are provided for producing transgenic Brassica plants involving the introduction of a DNA construct by microprojectile bombardment into pre-incubated microspores, microspore-derived embryos and microspore-derived hypocotyls. The methods find use in the development of improved agricultural varieties of Brassica plants through the incorporation of desirable agronomic traits.

L'invention concerne la manipulation génétique de plantes, particulièrement de plantes du genre Brassica. Des procédés sont mis à disposition pour la production de plantes de Brassica transgéniques impliquant l'introduction d'un ADN construit par un bombardement de microprojectiles dans des microspores incubées par avance, des embryons dérivés de microspores et des hypocotyles dérivés de microspores. Les procédés trouvent une utilisation dans le développement de variétés agricoles améliorées de plantes de Brassica grâce à l'incorporation de caractéristiques agronomiques souhaitables.

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