Micropropagation and production of phytopharmaceutical plants

Details Micropropagation and production of phytopharmaceutical plants Micropropagation and production of phytopharmaceutical plants

A - Human Necessities

01

H

A01H 4/00 (2006.01)

Patent

CA 2367957

The development of an in vitro regeneration system that utilizes a plant growth regulator having cytokinin activity for the induction of de novo shoots or somatic embryos on explants of phytopharmaceutical plants is provided. Transfer of the regenerated shoots or somatic embryos into a solid or liquid medium with no plant growth regulators results in the rapid and prolific growth of viable plantlets. The method and its modifications are intended for application to all phytopharmaceutical plants, in particular St.John's wort (Hypericum perforatum cv. Anthos), Huang-qin (Scutellaria baicalensis), Echinacea sp., Feverfew (Tanacetum parthenium), garlic (Allium sp.) and the like. Furthermore, a process for the uptake of nutrients, minerals or additives from the growth medium and accumulation of these in the consumable biomass of plants, hereafter referred to as phytofortification, is also described. This process provides additives within a bioavailable form within plants and renders nutrients and additives amenable for easy assimilation by the human of livestock digestive systems.

Cette invention concerne l'élaboration d'un système de régénération in vitro dans lequel on utilise un régulateur de la croissance végétale présentant une activité cytokinine pour provoquer l'apparition de pousses ou d'embryons somatiques de novo sur des explants de plantes médicinales. Le transfert de pousses ou d'embryons somatiques régénérés dans un milieu solide ou liquide exempt de régulateur de croissance végétale déclenche un développement rapide et prolifique de plantules viables. Ces procédé et les modifications qu'il induit conviennent pour toutes la palette phytopharmaceutique, en particulier l'herbe de la St Jean (Hypericum perforatum cv. Anthos), Huang-qin (Scutellaria baicalensis), Echinacea sp., la grande camomille (Tanacetum parthenium), l'ail (Allium sp.) etc. Est également décrit un procédé, dit de phytorenforcement, qui concerne l'assimilation de nutriments, de minéraux et d'additifs pris dans le milieu de croissance et leur accumulation dans la biomasse consommable des plantes. Ce procédé permet d'obtenir des additifs sous une forme biodisponible dans les végétaux et de rendre les nutriments et additifs facilement assimilables par les systèmes digestifs de l'homme et du bétail.

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