Microsatellite sequences for canine genotyping

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2233319

Methods of genotyping canines by analysis of polymorphisms in the number of microsatellite DNA repeats are provided. The internal repeat sequence is amplified by the use of specific primers. The number of repeats, and therefore the distance between the primers, is highly variable in a population, thereby providing an allelic marker for the locus. The combined information from multiple loci provides a means of distinguishing individuals, even among inbred dog breeds, for parentage testing, forensic testing and analysis of individuals relatedness.

Cette invention se rapporte à des procédés de détermination du génotype de races canines par analyse des polymorphismes au sein de l'ensemble des séquences de répétition microsatellites d'ADN. On amplifie la séquence de répétition interne au moyen d'amorces spécifiques. Le nombre de répétitions, et par conséquent la distance entre les amorces, varie considérablement au sein d'une population, ce qui permet de disposer d'un marqueur allèle pour un locus donné. Les informations combinées de multiples loci permettent d'effectuer une distinction entre des individus, même au sein de races canines consanguines, et elle servent à la vérification de paternité, aux expertises légales et à l'analyse des liens de parenté entre individus.

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