Microscale affinity purification system

G - Physics – 01 – N

Patent

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Details

G01N 1/34 (2006.01) G01N 1/40 (2006.01) G01N 33/561 (2006.01)

Patent

CA 2456078

A microscale affinity purification system has a plurality of capillary channels (100) which begin and end in common compartments (110, 120). An introduction cross-capillary channel (130) runs across the capillary channels (100) in a serpentine pattern (136, 138) near one end and a collection channel (140) similarly crosses the capillaries (100) in a serpentine pattern (148, 150) near the other end. A target molecule is introduced from a reservoir (132) which binds to a desired strong ligand in a sample in the capillaries (100). The target-strong ligand comlex migrates through the capillaries (100), is detected at a detector (146), and collected in a collection reservoir (144).

L'invention concerne un système de purification par affinité à échelle microscopique comportant une pluralité de canaux capillaires (100), commençant et finissant dans des compartiments communs (110, 120). Un canal transcapillaire d'introduction (130) traversent les canaux capillaires (100) en formant un motif de serpentin (136, 138), à proximité d'une extrémité, et un canal de recueillement (140) traverse de manière similaire les capillaires (100), en formant un motif de serpentin (148, 150), à proximité de l'autre extrémité. Une molécule cible est introduite à partir d'un réservoir (132), laquelle molécule se lie à un ligand fort voulu au niveau d'un échantillon, dans les capillaires (100). Le complexe cible-ligand fort migre à travers les capillaires (100), est détecté au niveau d'un détecteur (146), et est recueilli dans un réservoir de recueillement (144).

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