Microspheres with fluorescent spherical zones

Details Microspheres with fluorescent spherical zones Microspheres with fluorescent spherical zones

G - Physics

01

N

G01N 21/64 (2006.01) C08J 7/12 (2006.01) C40B 70/00 (2006.01) G01M 11/00 (2006.01) G01N 15/10 (2006.01) G01N 33/58 (2006.01) G02B 27/22 (2006.01) G12B 21/06 (2006.01)

Patent

CA 2218483

The invention describes novel fluorescent microspheres that are labeled so as to possess at least one fluorescent spherical zone, such that following excitation of the particle at an appropriate wavelength, an equatorial cross-section displays a distinct fluorescent ring that is concentric with and within said microsphere. The invention also describes the method of preparing the novel microspheres, the method of calibrating microscopy instrumentation using the novel microspheres, the method of using the novel microspheres as distinct labels for combinatorial analysis and the use of the labeled microspheres as tagging agents and tracers.

L'invention décrit de nouvelles microsphères fluorescentes, marquées de façon à comporter au moins une zone sphérique fluorescente, afin qu'après l'excitation de la particule à une longueur d'onde appropriée, une section transversale équatoriale présente un anneau fluorescent distinct, concentrique à la microsphère et à l'intérieur de celle-ci. L'invention décrit également la méthode pour préparer les nouvelles microsphères, une méthode d'étalonnage des instruments de microscopie utilisant les nouvelles microsphères, une méthode pour utiliser les nouvelles microsphères comme marqueurs distincts dans les analyses combinatoires, et l'emploi des microsphères marquées comme traceurs et agents d'identification.

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