Microtiter plate (mtp) based high throughput screening (hts)...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/00 (2006.01) C07K 14/32 (2006.01) C07K 14/37 (2006.01) C12M 1/18 (2006.01) C12N 9/28 (2006.01) C12N 9/42 (2006.01) C12N 9/54 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2388982

The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. In a first aspect the invention relates to a method for high throughput screening (HTS) of a large population of host cells for production of a molecule of interest, the method comprising the steps of: a) arranging the host cells in a spatial array so each position in the spatial array is occupied by one cell, b) cultivating the host cells under growth conditions suitable for HTS, c) assaying each array position for production of the molecule of interest, and d) selecting the cells from those positions where the molecule was produced, as determined in step c).

La présente invention concerne une méthode permettant de réaliser des analyses destinées au criblage efficace et précis de grands nombres de populations cellulaires produisant des variants d'une molécule considérée. Selon un premier aspect, l'invention concerne une méthode de criblage à haut rendement réalisée sur une population importante de cellules hôtes et visant à détecter la production d'une molécule considérée, laquelle méthode consiste: a) à disposer les cellules hôtes selon un certain agencement spatial, de manière que chaque position dudit agencement spatial soit occupée par une seule cellule; b) à cultiver les cellules hôtes dans des conditions de croissance se prêtant à un criblage à haut rendement; c) à analyser chaque position de l'agencement pour y détecter la production de la molécule considérée; et d) à sélectionner les cellules provenant des positions où ladite molécule a été produite, tel que déterminé en c).

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