Microvessel cell isolation method and apparatus

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/00 (2006.01) C12M 3/08 (2006.01) C12S 3/24 (2006.01)

Patent

CA 2164416

A processing vessel (12) for isolating microvessel endothelial cells from liposuctioned fat tissues includes a fat-receiving basket (54) defined by polyester screen material. Fat tissue removed from a patient by liposuction is received into the basket (54) and is rinsed and digested with an enzymatic solution. The freed microvessel endothelial cells from the fat tissues are separated from the fat cells, and from blood cells and other materials which may be present in the basket by centrifuging. A bottom chamber (62) of the processing vessel (12) is configured to define a "pellet" of isolated endothelial cells which may be removed from the processing vessel (12) for deposition on the inner lumenal surface of a synthetic graft which the fat-donor patient is to receive.

Un récipient de traitement permettant d'isoler des cellules endothéliales microvasculaires à partir de tissus adipeux obtenus par liposuccion comprend un panier de réception de graisse défini par un matériau de criblage à base de polyester. Les tissus adipeux enlevés d'un patient par liposuccion sont reçus dans le panier, puis rincés et digérés au moyen d'une solution enzymatique. Les cellules endothéliales microvasculaires libérées des tissus adipeux sont séparées par centrifugation des cellules de graisse et des cellules sanguines et des autres substances pouvant être présentes dans le panier. Une chambre inférieure du récipient de traitement est configurée de façon à définir une "pastille" de cellules endothéliales isolées qui peut être enlevée du récipient de traitement afin d'être déposée sur la surface interne d'un passage d'un greffon synthétique que le patient donneur de graisse doit recevoir.

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