Microwave method for preparation of serum samples for b-12...

Details Microwave method for preparation of serum samples for b-12... Microwave method for preparation of serum samples for b-12...

G - Physics

01

N

150/14, 167/37

G01N 33/82 (2006.01) G01N 29/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2008473

17 MICROWAVE METHOD FOR PREPARATION OF SERUM SAMPLES FOR B-12 AND FOLATE ASSAYS ABSTRACT OF THE DISCLOSURE The present invention provides novel processes for the release of vitamin B12 and/or folate in mammalian serum samples from endogenous serum binders comprising microwaving the serum samples prior to assaying the serum samples for vitamin B12 and/or folate. Prior to the assay procedure, the serum samples are typically microwaved for about 20-90 seconds, most preferably for about 60 seconds at a high setting or such that the temperature of the serum samples is brought to a temperature of about 65 to 100°C, most preferably 80-85°C, for at least 15 seconds.

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