C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/36
C12N 9/64 (2006.01) A23C 19/04 (2006.01)
Patent
CA 1178223
ABSTRACT OF THE DISCLOSURE A high potency rennet substitute from seals is provided herein. Such extract closely matches the characteristics of the calf rennet, namely, (1) is coagulates milk over a wide range from 6.0 to 6.8, (2) it has a high ratio of milk clotting to proteolytic activity, (3) it has limited action on milk proteins, (4) it has limited substrates specificity as evidenced by its relative inability to inactivate ribonuclease - a test used to distin- guish renning from other proteolytic enzymes; and (5) it results in a desir- able aged flavor in cheddar cheese. This crude preparation of seal pro- teolytic enzyme precursors is composed of 4 components, the major two of which have molecular weights of 28,500 and 40,000 Daltons as determined by gel filtration on a SEPHADEX G-100 (Trade Mark for a polysacchoride dextran in which the functional ionic groups are linked to the glucose units of the polysacchoride chains by ether exchanges) column and 40,000 and 50,000 Daltons by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The crude active preparation has a pH optimum of 2.6 for hemoglobin. hydrolysis at 30°C. Results of an isoelectric point determination revealed that the four components of the active preparation have isoelectric points of 4.9, 4.3, 3.8 and 3.4.
388552
Haard Norman F.
Shasuzzaman Kazi M.
Borden Ladner Gervais Llp
Haard Norman F.
Shasuzzaman Kazi M.
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