C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/52 (2006.01) A23C 19/032 (2006.01) C12N 9/54 (2006.01) C12P 21/06 (2006.01)
Patent
CA 2491463
It is intended to provide a milk-coagulating enzyme originating in a bacterium and a process for producing cheese by using this enzyme. Namely, an enzyme which is produced by a bacterium belonging to the genus Paenibacillus sp. and has an activity of coagulating milk, characterized by having the following enzymological properties: (1) function: having an activity of coagulating milk to form a curd; (2) substrate specificity: acting on .kappa.-casein as a substrate and specifically cleaving Thr94-Met95 in the presence of calcium; (3) optimum pH: 6.0 to 7.0; and (4) molecular weight: 35,000 to 37,000 Da when measured by SDS-PAGE; a process for producing the enzyme; a process for producing cheese; and a cheese-like food product.
L'invention concerne une enzyme de coagulation du lait provenant d'une bactérie et un procédé de fabrication de fromage à l'aide de cette enzyme. Cette enzyme, qui est produite par une bactérie du genre Paenibacillus sp.,présente une activité de coagulation du lait et se caractérise par les propriétés enzymologiques suivantes : (1) fonction : activité de coagulation du lait donnant naissance à un caillé ; (2) spécificité du substrat : action sur la caséine K comme substrat et spécifiquement, clivage de Thr94-Met 95 en présence de calcium ; (3) pH optimum : 6.0 à 7,0 ; et (4) poids moléculaire : 35 000 à 37 000 Da mesuré selon SDS-PAGE. L'invention concerne également un procédé d'obtention de cette enzyme, une méthode de fabrication du fromage et une denrée alimentaire de type fromage.
Kawakami Makoto
Miyashita Hiroko
Miyashita Shuhei
Nagashima Koji
Nakagawa Ryoji
Mahoroba Co. Ltd.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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