Minicircle vector production

Details Minicircle vector production Minicircle vector production

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01)

Patent

CA 2524057

A plasmid is provided comprising comprising the following func~tional units: a prokaryotic origin of replication, a marker sequence, two specific recombinase recognition sequences and a multiple cloning site, whereby it comprises a gene coding for a sequence specific recombinase, whereby the units are arranged on the plasmid in such a way that the plasmid is divided into a miniplasmid and a minicircle upon expression of the sequence specific recombinase, said miniplasmid comprising the prokaryotic origin of replication, the marker sequence and the gene for the sequence specific recombinase and said minicircle comprising the multiple cloning site.

La présente invention concerne un plasmide comprenant les unités fonctionnelles suivantes : - une origine procaryote de réplication une séquence de marqueur deux séquences de reconnaissance de recombinase spécifiques et un site de clonage multiple. Ce plasmide comprend un gène qui code une recombinase spécifique de séquence. Les unités sont situées sur le plasmide de façon que ce plasmide est divisé en un miniplasmide et en un minicercle par l'expression de la recombinase spécifique de séquence. Ce miniplasmide comprend l'origine procaryote de réplication, la séquence de marqueur et le gène pour la recombinase spécifique de séquence et le minicercle comprend le site de clonage multiple.

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