Minimizing metal toxicity during electroporation enhanced...

C - Chemistry – Metallurgy – 12 – N

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C12N 13/00 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2479028

Methods are provided for introducing a polynucleotide into healthy tissue and generating a pulsed electric field in the tissue via invasive electrodes, resulting in enhanced delivery of the polynucleotide into cells of the tissue, while minimizing local side effects to the electroporated tissue and systemic side effects to the electroporated organizm due to metal contaminants released from said electrodes. In one embodiment, the invention methods Use electrodes of gold, gold alloys or other metal that minimize the introduction of toxic amounts of the metal into electroporated tissue. In other embodiments, the invention methods are utilized for the gene therapy by administering DNA to cells of suitable target tissue, and for the induction of an immune response by administration of a DNA vaccine.

L'invention porte sur un procédé d'introduction d'un polynucléotide dans un tissu sain et de production d'un champ électrique dans ledit tissu à l'aide d'électrodes invasives. Il en résulte un meilleur transfert du polynucléotide aux cellules du tissu et une réduction des effets secondaires locaux dans le tissu électroporé, et des effets secondaires systémiques dans l'organisme électroporé, dus aux contaminants métalliques libérés par lesdites électrodes. Dans une exécution on utilise des électrodes d'or, d'alliages d'or ou d'autres métaux qui réduisent l'apport de quantités toxiques de métal dans le tissu. Dans d'autres exécutions, les procédés de l'invention sont utilisés en thérapie génique pour administrer de l'ADN aux cellules de tissus cibles adéquats, afin d'y induire une réponse immunitaire due à l'administration d'un vaccin d'ADN.

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