Mites-like element and transcriptional activation element

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2379857

The invention provides novel, carrot-derived MITE-like elements (transposable elements). It further provides transcriptional activation elements comprising at least one transposable element, in particular one of the above MITE-like elements. Specifically, it provides transcriptional activation elements having a DNA comprising the nucleotide sequence shown under SEQ ID NO:1 or a functional equivalent thereto and/or a DNA comprising the nucleotide sequence shown under SEQ ID NO:2 or a functional equivalent thereto. The transcriptional activation elements of the invention can increase or activate the reduced expression of a foreign gene introduced by the transgenic technology. Therefore, the transcriptional activation elements contribute to stable expression of a foreign gene introduced in a plant genome and are useful in stably producing genetically modified plants.

L'invention concerne de nouveaux éléments de type MITE (éléments transposables) dérivés de la carotte. Elle concerne également des éléments d'activation transcriptionnels comprenant au moins un élément transposable, en particulier un des éléments de type MITE précités. Elle porte en particulier sur des éléments d'activation transcriptionnels dont l'ADN comprend la séquence de nucléotides présentée dans SEQ ID NO:1 ou un équivalent fonctionnel, et/ou un ADN comprenant la séquence de nucléotides présentée dans SEQ ID NO:2 ou un équivalent fonctionnel. Les éléments d'activation transcriptionnels de l'invention permettent d'augmenter ou d'activer l'expression d'un gène étranger introduit grâce à la technique transgénique. Ainsi, les éléments d'activation transcriptionnels contribuent à stabiliser l'expression d'un gène étranger introduit dans un génome végétal, et à produire de manière stable des végétaux génétiquement modifiés.

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