Mitotic index assay

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/34 (2006.01)

Patent

CA 2613174

Mitosis of cells is determined, particularly in the presence of a candidate agent, using cells comprising members of an enzyme fragmentation complex pair, where one of th members is in the nucleus and the other member is in the cytoplasm. By growing the cells where mitosis may occur, one adds a substrate providing a detectable product, where the production of the detectable product is indicative of mitosis.

La mitose de cellules est déterminée, notamment en présence d'un agent candidat, au moyen de cellules comportant des éléments d'une paire complexe de fragmentation d'enzymes, où un des éléments se trouve dans le noyau et l'autre élément se trouve dans le cytoplasme. Grâce à la croissance de cellules où la mitose peut se produire, on ajoute un substrat fournissant un produit détectable, où la production du produit détectable est indicative de la mitose.

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