C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) B01J 41/20 (2006.01) B01J 47/04 (2006.01)
Patent
CA 2372521
Mixed-bed solid phases are provided, with methods for using such solid phases to isolate target nucleic acids, such as plasmid DNA, chromosomal DNA, RNA, or nucleic acids generated by enzymatic amplification from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. The mixed-bed solid phases of this invention are mixtures of at least two different solid phases, each of which has a capacity to bind to the target nucleic acid under different solution conditions, and the capacity to release the nucleic acid under similar elution conditions. By exchanging solution conditions according to the methods of this invention, one can remove contaminants from the target nucleic acid bound to the mixed-bed solid phase, then elute the target nucleic acid in an elution buffer.
L'invention concerne des phases solides à lits mélangés, et des méthodes mettant en oeuvre lesdites phases solides pour isoler des acides nucléiques cibles, tels que l'ADN plasmidique, l'ADN chromosomique, l'ARN ou des acides nucléiques produits par amplification enzymatique à partir de contaminants, notamment des protéines, des lipides, des fragments cellulaires ou autres acides nucléiques. Les phases solides à lits mélangés de l'invention sont des mélanges d'au moins deux phases solides différentes, dont chacune a la faculté de se lier à l'acide nucléique cible dans différentes conditions de solution, ainsi que la capacité de libérer l'acide nucléique dans des conditions d'élution similaires. En procédant à un échange des conditions de solution selon les méthodes de l'invention, on peut éliminer les contaminants de l'acide nucléique cible lié à la phase solide à lits mélangés, puis éluer l'acide nucléique cible dans un tampon d'élution.
Bitner Rex M.
Grosch Josephine C.
Holmes Diana L.
Katzhendler Jehoshua
Simpson Daniel J.
Promega Corporation
Shapiro Cohen
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