Mixed cell populations for tissue repair and separation...

A - Human Necessities – 61 – K

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

A61K 35/12 (2006.01) A61P 9/10 (2006.01)

Patent

CA 2668330

The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti- inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use. In such embodiments, the final composition is a substantially purified and concentrated cell mixture suspended in a physiologic solution suitable for immediate use in humans without further washing, volume reduction, or processing. Embodiments are also applicable to harvesting (and/or washing) particles within a liquid or solution within a chamber.

Technique de culture cellulaire à échange fluidique et cellules de réparation tissulaire résultantes, ainsi que procédés relatifs à l'utilisation de telles cellules. On décrit un procédé qui comprend une nouvelle étape de lavage augmentant les propriétés de réparation tissulaire de ces cellules. Ladite étape permet la production de populations des cellules considérées ayant des capacités accrues de réparation tissulaire et antiinflammatoires. On présente des variantes qui proposent un procédé post-culture pour des cultures cellulaires comprenant généralement les étapes suivantes: lavage visant à éliminer les éléments de culture résiduels non désirés, réduction de volume, et récolte visant à retirer les cultures cellulaires. De préférence, toutes ces étapes sont conduites dans une chambre de culture cellulaire close aseptisée selon une technique de séparation qui réduit au minimum la rupture cellulaire mécanique et est simple à automatiser. Les cellules récoltées peuvent ensuite être concentrées en un volume final pour l'utilisation prévue. Sous de telles variantes, la composition finale est un mélange sensiblement purifié et concentré en suspension dans une solution physiologique se prêtant à une utilisation immédiate chez l'homme sans autre lavage, réduction de volume ou traitement. Les variantes considérées s'appliquent aussi à la récolte (et/ou au lavage) de particules dans un liquide ou une solution à l'intérieur d'une chambre.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Mixed cell populations for tissue repair and separation... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mixed cell populations for tissue repair and separation..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mixed cell populations for tissue repair and separation... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1397499

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.