Mixing and matching tc proteins for pest control

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01N 63/02 (2006.01) C07K 14/195 (2006.01) C07K 14/24 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2514041

The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein.

L'invention concerne les protéines de type complexe toxine (TC), que l'on obtient à partir de Xenorhabdus, Photorhabdus et Paenbacillus et que l'on peut utiliser indifféremment. Dans des modes de réalisation particulièrement préférés, la toxicité d'une protéine TC autonome ( de Xenorhabdus, Photorhabdus ou Paenbacillus, par exemple) est renforcée par une ou plusieurs protéines TC <= potentialisatrices >= dérivées d'un organisme source d'un genre différent dont on a dérivé la toxine. L'intérêt réside dans les importantes implications des types individuels de protéines TC et l'extension de la portée des services que les spécialistes leur reconnaîtront. On pourra désormais utiliser un ensemble simple de potentialisateurs pour renforcer l'activité d'une toxine de protéine Xenorhabdus autonome ainsi que d'une toxine de protéine Photorhabdus autonome. (Les protéines à toxines Xenorhabdus étant plus recherchées pour combattre les lépidoptères, et les protéines à toxines Photorhabdus pour combattre les coléoptères). On réduit ainsi le nombre de gènes et d'événements de transformation, dont l'expression par une plante transgénique est nécessaire pour que la lutte contre un spectre plus large de ravageurs nuisibles soit efficace. L'invention concerne également certaines combinaisons préférées de protéines TC hétérologues. L'invention concerne enfin d'autres objets, avantages et caractéristiques dont l'évidence apparaîtra aux spécialistes dans ce domaine.

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