Mobility-modifying cyanine dyes

C - Chemistry – Metallurgy – 09 – B

Patent

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Details

C09B 23/02 (2006.01) C07F 9/24 (2006.01) C07H 21/00 (2006.01) G01N 27/447 (2006.01) G01N 33/52 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2396067

The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the heterocyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence- based 4-color nucleic acid sequencing reactions.

L'invention concerne une nouvelle catégorie de composants à colorant à base de cyanine fluorescents modifiés au niveau de l'un des atomes d'azote de la chaîne hétérocyclique au moyen d'une fraction modificatrice de mobilité permettant d'ajuster ou de régler les mobilités électrophorétiques des polynucléotides marqués avec des colorants à base de cyanine à modification de mobilité, de manière prévisible mais sans élimination de l'activité enzymatique. La possibilité de régler de manière prévisible les mobilités électrophorétiques relatives des colorants permet d'établir des séries de colorants fluorescents adaptés en mobilité, obéissant à une variété de structures, pour les besoins d'une variété d'applications, y compris les réactions de séquençage d'acides nucléiques à quatre couleurs en fluorescence.

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