Modification of flavonoid biosynthesis in plants

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) C12N 15/53 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2615949

The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, flavonoid-regulating transcription factors and a flavonoid-specific membrane transporter in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the protein involved in flavonoid biosynthesis is selected from the group consisting of TRANSPARENT TESTA 12 (TT12), TRANSPARENT TESTA GLABRA 1 (TTG1), TRANSPARENT TESTA 2 (TT2), TRANSPARENT TESTA 8 (TT8), leucoanthocyanidin dioxygenase (LDOX), cinnamate-4-hydroxylase (C4H), 4- coumaroyl:CoA-ligase (4CL); and functionally active fragments and variants thereof.

La présente invention concerne des acides nucléiques codant pour des enzymes de la biosynthèse des flavonoïdes, des facteurs de transcription de la régulation des flavonoïdes et un transporteur de membrane spécifique aux flavonoïdes chez les plantes et leur utilisation pour la modification de la biosynthèse des flavonoïdes chez les plantes. La présente invention concerne également des gènes hybrides et des vecteurs incluant de tels acides nucléiques et des polypeptides associés. Plus particulièrement, la protéine impliquée dans la biosynthèse des flavonoïdes est choisie parmi le groupe formé de TRANSPARENT TESTA 12 (TT12), TRANSPARENT TESTA GLABRA 1 (TTG1), TRANSPARENT TESTA 2 (TT2), TRANSPARENT TESTA 8 (TT8), leucoanthocyanidine dioxygénase (LDOX), cinnamate-4-hydroxylase (C4H) et 4-coumaroyl:CoA-ligase (4CL) ; et des fragments fonctionnellement actifs et des variants de ceux-ci.

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