Modification of multidomain enzyme for expression in plants

Details Modification of multidomain enzyme for expression in plants Modification of multidomain enzyme for expression in plants

C - Chemistry, Metallurgy

12

N

C12N 9/34 (2006.01) C12N 9/42 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2749356

Compositions and methods for expressing a multidomain enzyme in a plant are provided. The compositions include plants, seeds, plant tissues, and plant parts expressing a modified multidomain enzyme enzyme. The modified multidomain enzyme has a heterologous linker region that is not cleaved when the modified multidomain enzyme is expressed in a plant. In various embodiments, the linker region comprises the sequence set forth in SEQ ID NO: 18, 19, or 20. Further provided are methods for producing a modified multidomain enzyme enzyme comprising cultivating plants expressing the modified multidomain enzyme. Downstream uses of transgenic plant material of the invention include agronomical and industrial uses, for example, human food, animal feed, pharmaceuticals, biofuel, industrial alcohol, fermentation feedstocks, and the like.

L'invention porte sur des compositions et des procédés pour l'expression d'une enzyme multidomaine dans une plante. Les compositions comprennent des plantes, des graines, des tissus végétaux et des parties de plante exprimant une enzyme multidomaine modifiée. L'enzyme multidomaine modifiée a une région liante hétérologue qui n'est pas clivée lorsque l'enzyme multidomaine modifiée est exprimée dans une plante. Dans divers modes de réalisation, la région liante comprend la séquence établie dans SEQ ID NO : 18, 19 ou 20. L'invention porte en outre sur des procédés de production d'une enzyme multidomaine modifiée comprenant la culture de plantes exprimant l'enzyme multidomaine modifiée. Des utilisations en aval de matériel végétal transgénique de l'invention comprennent des utilisations agricoles et industrielles, par exemple, l'alimentation humaine, l'alimentation animale, les produits pharmaceutiques, le biocarburant, l'alcool industriel, les charges d'alimentation de fermentation et similaires.

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