Modification of plant phenotypes through tor gene expression

Details Modification of plant phenotypes through tor gene expression Modification of plant phenotypes through tor gene expression

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01) C12Q 1/00 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2745852

Broad experimental tools that include biochemical, molecular, developmental, global genomics and loss and gain of function transgenic approaches have been applied to address target of rapamycin (TOR) signaling pathway in plants, especially using Arabidopsis model system and Brassica napus crop. Towards this objective, putative TOR interacting proteins (TIPs) have been identified and functions of these implicated in diverse developmental and biochemical processes have been investigated. Functional studies including over-expression and silencing of TOR and TIPs have shown a range of phenotypes that include embryo defective, nutrition-use-efficiency, water-use- efficiency and stress resistance in transgenic Arabidopsis and Brassica lines. Some of these phenotypes are relevant to important developmental pathways implicated in canola crop yield and performance.

L'invention concerne des outils expérimentaux généraux comprenant la génomique biochimique, moléculaire, expérimentale, globale et les approches transgéniques de perte de gain des fonctions ont été appliquées pour traiter la cible de la voie de signalisation de la rapamycine (TOR) chez les plantes, en utilisant en particulier le système du modèle Arabidopsis et la culture de Brassica napus. Pour atteindre cet objectif, les protéines d'interaction TOR putatives (TIP) ont été identifiées, et leurs fonctions impliquées dans divers procédés expérimentaux et biochimiques ont été étudiées. Des études fonctionnelles comprenant la surexpression et la mise sous silence des TOR et des TIP ont montré un éventail de phénotypes comprenant l'efficacité défectueuse de l'utilisation de la nutrition embryonnaire, l'efficacité de l'utilisation de l'eau et la résistance aux contraintes dans les lignes transgéniques Arabidopsis et Brassica. Certains de ces phénotypes concernent les voies de développement importantes impliquées dans le rendement et les performances des cultures de canola.

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