Modification of plasminogen activators

C - Chemistry – Metallurgy – 12 – N

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195/127, 195/39.

C12N 9/72 (2006.01) A61K 38/49 (2006.01) C07K 16/40 (2006.01) C12N 9/64 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2031210

ABSTRACT MODIFICATION OF PLASMINOGEN ACTIVATORS Phosphorylated plasminogen activator, such as phosphorylated pro- urokinase (pro-u-PA), which is substantially free from unphospho- rylated plasminogen activator, may be obtained by phosphorylating unphosphorylated plasminogen activator with a phosphorylating enzyme or by separating phosphorylated plasminogen activator from a mixture of phosphorylated plasminogen activator and unphosphorylated plas- minogen activator. Phosphorylated pro-u-PA, which is substantially free from unphosphorylated pro-u-PA, is converted by plasmin into phosphorylated u-PA. The phosphorylated plasminogen activators such as phosphorylated pro-u-PA, u-PA and t-PA are useful as thrombolytic agents. 328807BA.001/A26/AS/KPJ/KPJ/10.4.1990

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