Modification of starch biosynthetic enzyme gene expression...

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C08B 30/00 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2330213

The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination; and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.

L'invention porte sur l'utilisation d'un clone d'ADNc pour construire des gènes sens et antisens inhibant dans le blé l'activité enzymatique de la synthase de l'amidon. L'invention porte plus spécifiquement sur un procédé de régulation de la structure fine de l'amidon de grains de céréales consistant: (1) à préparer un gène chimère comportant un fragment d'acide nucléique codant pour le gène structurel de la synthase d'amidon, ou l'un de ses fragments, fonctionnellement lié dans l'orientation sens ou antisens par le côté amont à un fragment d'acide nucléique codant pour un promoteur commandant l'expression de gènes dans les tissus de l'endosperme du blé, et fonctionnellement lié par le côté aval à un fragment d'acide nucléique codant pour une séquence régulatrice idoine de terminaison transcriptionnelle; et (2) à transformer les cultures de céréales à l'aide dudit gène chimère dont l'expression modifie la structure fine de l'amidon du grain desdites cultures transformées, par comparaison avec celle de cultures ne possédant pas ledit gène chimère.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Modification of starch biosynthetic enzyme gene expression... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Modification of starch biosynthetic enzyme gene expression..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Modification of starch biosynthetic enzyme gene expression... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1428760

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.